Isolation of <i>Malassezia</i> yeasts from dogs with gastrointestinal disease undergoing duodenal endoscopy

Objectives To culture Malassezia and other fungi from the duodenum of dogs with gastrointestinal signs undergoing routine endoscopic examination. Materials Methods Quantitative microbial was performed on duodenal juice aspirated suspected enteropathy during upper endoscopy; samples were cultured Sabouraud's dextrose agar (30, 32 37°C) modified Dixon (32°C) for 14 days. Isolates identified phenotypically by matrix-assisted laser desorption ionisation-time flight, internal transcribed spacer sequencing. Yeast presence also evaluated cytological histopathological examination smears biopsy specimens. Results Forty-five recruited chronic inflammatory (n=38), granulomatous colitis (n=2), gastric adenocarcinoma small cell lymphoma (n=1) idiopathic severe haemorrhage (n=2). Fungi dogs: pachydermatis isolated eight [chronic (n=7) (along Candida albicans n=1); (n=1)] sympodialis another (gastric adenocarcinoma). Five yielded yeasts (C. albicans, glabrata, Kazachstania slooffiae, telluris, Pichia kudriavzevii [syn. C. krusei]). Yeasts never observed in Fluorescent microscopical specimens showed yeast only one case, which K. slooffiae subsequently isolated. Clinical Significance Based a literature search, this is first report isolation M. pachydermatis, sympodialis, telluris canine duodenum. Further studies are needed to determine whether these resident or transient their has pathogenic effect host.